PCR in real time

RealBest diagnostic kits are intended for the detection of infectious agents and determination of genetic polymorphisms by means of real-time PCR. In-house developed technologies turn the real-time PCR analysis procedure from a complicated process, which requires extensive specialized knowledge and practical experience, into a standardized procedure that is simple and easy to perform.

AD Vector-Best offers simple user solutions for diagnostics, such as lyophilized ready mixes with a long shelf life and a transport temperature of up to 26 °C.

RealBest kits
  • A complex approach to PCR diagnostics that includes sample preparation, PCR and evaluation of results
  • Simple extraction of nucleic acids using magnetic particles that enable a much better yield
  • Freeze-dried ready-to-use master mix for real-time DNA/RNA detection, no aliquoting or spilling required
  • Compatible PCR and RT-PCR protocols for the detection of a wide range of pathogens
  • Non-competitive internal control
  • Storage of all components at the same temperature (2-8)°C for up to 72 months; no freezing required
  • Transport at a temperature of up to 26°C for no longer than 70 days is acceptable
  • In terms of quality and ease of use, they are completely suitable for our laboratories, and their affordable price makes them available to everyone on the market
 
Different formats

Str-format: Ready Master Mix is ​​a lyophilized mixture in 0.2 ml white tubes in a 96-tube pack. The only step required for the reaction procedure is the addition of the extracted DNA/RNA samples.

Uni-format: Ready Master Mix is ​​a lyophilized mixture in transparent tubes with a volume of 0.2 ml. Add the extra captured DNA/RNA to the tubes and run the test. One tube – one reaction.

Real-time PCR systems and formats

Str format may be used with: CFX96™ (Bio-Rad, USA), DTprime (DT-96), DTlite (DNA-Technology, Russia), FLUORITE (Xi’an TianLong, Science and Technology Co., Ltd., China).

Uni format may be used with: CFX96™ (Bio-Rad, USA), DTprime (DT-96), DTlite (DNA-Technology, Russia), Rotor-Gene® Q (Qiagen, Germany), Rotor-Gene® 6000/3000 ( Corbett Research, Australia), FLUORITE (Xi’an TianLong, Science and Technology Co., Ltd., China).